Vol. 22 No. 2 (2023)
Original Articles

Insulin-like growth factor-1 short-period therapy improves Non-Alcoholic Fatty Liver Disease (NAFLD) in obese mice

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  • Daniela C. Andrade,
  • Filipe Jorge Nascimento,
  • Genilza P. de Oliveira,
  • Thiago Freire,
  • Simone N. de Carvalho,
  • Ana Carolina Stumbo,
  • Laís Carvalho,
  • Alessandra A. Thole,
  • Erika Cortez
Daniela C. Andrade
Bio
Filipe Jorge Nascimento
Bio
Genilza P. de Oliveira
Bio
Thiago Freire
Bio
Simone N. de Carvalho
Bio
Ana Carolina Stumbo
Bio
Laís Carvalho
Bio
Alessandra A. Thole
Bio
Erika Cortez
Bio
DOI: https://doi.org/10.12957/bjhbs.2023.79959

Published 2023-12-20

Keywords

  • Non-Alcoholic Fatty Liver Disease,
  • Liver Fibrosis,
  • IGF-1,
  • Obesity

How to Cite

1.
Daniela C. Andrade, Nascimento FJ, P. de Oliveira G, Freire T, Simone N. de Carvalho, Ana Carolina Stumbo, Laís Carvalho, Alessandra A. Thole, Erika Cortez. Insulin-like growth factor-1 short-period therapy improves Non-Alcoholic Fatty Liver Disease (NAFLD) in obese mice. BJHBS [Internet]. 2023 Dec. 20 [cited 2024 Apr. 27];22(2). Available from: https://bjhbs.hupe.uerj.br/bjhbs/article/view/151

Copyright (c) 2023 Brazilian Journal of Health and Biomedical Sciences

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Abstract

This study seeks to evaluate Insulin-like Growth Factor 1 (IGF-1) short-period therapy in Non-Alcoholic Fatty Liver Disease (NAFLD) as it relates to western diet-induced obesity. For this purpose, 21-day-old male Swiss mice were divided into a control group (CG, N=8), which was fed a standard diet, and an obese group (GO, N=16), which was fed a western diet, rich in saturated fat and simple carbohydrates, for 12 weeks. In the 11th week, part of the animals in the obese group (N=8) received a daily subcutaneous injection of recombinant human IGF-1 (100µg/kg/day) during seven consecutive days (GO+IGF-1). Biometric and metabolic parameters, intraperitoneal glucose tolerance test (IGTT), quantitative analysis of liver steatosis, quantitative analysis of collagen in liver and expression of immunoperoxidase of alpha-smooth muscle actin (a-SMA) were analyzed. Our data demonstrated that IGF-1 short-term treatment was able to improve obesity-related biometric and metabolic parameters. In addition, it promoted the recovery of liver parenchyma, thereby reducing steatosis and fibrosis, thus demonstrating an important hepatoprotective action. 

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