Vol. 24 No. 1 (2025)
Original Articles

Updates on the antimicrobial properties of garlic (Allium sativum) biomolecules in the treatment of human infectious diseases

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  • Marli do Carmo Cupertino,
  • Samara A. Cardoso-Nascimento ,
  • Rafaella N. Fortini-Duarte,
  • Adriano S. Barbosa-Castro,
  • Rodrigo Siqueira-Batista
Marli do Carmo Cupertino
Federal University of Viçosa
Bio
Samara A. Cardoso-Nascimento
Departamento de Medicina e Enfermagem, Universidade Federal de Viçosa. Viçosa, MG, Brazil.
Rafaella N. Fortini-Duarte
Federal University of Viçosa
Adriano S. Barbosa-Castro
Federal University of Viçosa
Bio
Rodrigo Siqueira-Batista
Federal University of Viçosa
Bio
DOI: https://doi.org/10.12957/bjhbs.2025.93428

Published 2025-08-08

Keywords

  • Phytotherapy,
  • antimicrobial activity,
  • allicin,
  • infection,
  • bacteria,
  • antibiotics,
  • natural compounds
    • ...More
    Less

How to Cite

1.
do C. Cupertino M, C. Nascimento SA, N. F. Duarte R, S. B. Castro A, Siqueira-Batista R. Updates on the antimicrobial properties of garlic (Allium sativum) biomolecules in the treatment of human infectious diseases. BJHBS [Internet]. 2025 Aug. 8 [cited 2025 Oct. 17];24(1):10-2. Available from: https://bjhbs.hupe.uerj.br/bjhbs/article/view/195

Copyright (c) 2025 Brazilian Journal of Health and Biomedical Sciences

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Abstract

Garlic (Allium Sativum) is the extensively consumed as seasoning, can be classified as a medicinal food, by the anti-inflammatory and antimicrobial properties. This study summarized the garlic biomolecules and up to date the main antimicrobial properties, correlating them with its phytochemical composition. This review article search for articles in SCOPUS, PubMed, and SciELO databases, using ((Anti-infective agents) OR (Antimicrobial)) AND (garlic) descriptors. Garlic is a good option to be associated with traditional medicines and emerges as a potential phytochemicals source. It has application in natura, aqueous and alcoholic extracts, and as essential oils. The macrostructure is divided into its roots, bulb, stem, clove and leaves. In the leaf, it was found the phenolic compounds and flavonoids, which are important constituents in the antimicrobial character. The allicin, the main bioactive component, is mostly located in the stem and leaves. The effects of garlic affect the metabolism, the stabilization of the plasma membrane, the adhesion of microorganisms, and influence on the formation of biofilms by bacteria, mainly Escherichia coli and the fungus Candida albicans. This study concludes that Allium sativum is an option in the potential antimicrobial phytochemicals development and an excellent contributor to strengthening the immune system.

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References

  1. Antimicrobial Resistance Collaborators. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet. 2022;399(10325):629–55. doi:10.1016/S0140-6736(21)02724-0.
  2. World Bank. Drug-resistant infections: a threat to our economic future [Internet]. Washington (DC): World Bank Group; 2017 [cited 2024 Feb 12]. Available from: https://www.worldbank.org/en/topic/health/publication/drug-resistant-infections-a-threat-toour-
  3. economic-future
  4. Jikah AN, Edo GI. Mechanisms of action by sulphur compounds in Allium sativum: a review. Pharmacol Res Mod Chin Med. 2023;2:100323. doi:10.1016/j.prmcm.2023.100323.
  5. Vinayagam R, Lee KE, Ambati RR, Gundamaraju R, Ramadan MF, Kang SG. Recent development in black garlic: nutraceutical applications and health-promoting phytoconstituents. Food Rev Int. 2023. doi:10.1080/87559129.2023.2217593.
  6. Indu MN, Hatha AAM, Abirosh C, Harsha U, Vivekanandan G. Antimicrobial activity of some of the South-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Braz J Microbiol. 2006;37:153–8. doi:10.1590/S1517-83822006000200011.
  7. Majewski M. Allium sativum: facts and myths regarding human health. Rocz Panstw Zakl Hig. 2014;65(1):1–8.
  8. Kyung KH, Lee YC. Antimicrobial activities of sulfur compounds derived from S-alk(en)yl-L-cysteine sulfoxides in Allium and Brassica. Food Rev Int. 2001;17(2):183–98.
  9. doi:10.1081/fri-100000268.
  10. Zugaro S, Benedetti E, Caioni G. Garlic (Allium sativum L.) as an ally in the treatment of nflammatory bowel diseases. Curr Issues Mol Biol. 2023;45(1):685–98. doi:10.3390/cimb45010046.
  11. Sasi M, Kumar S, Kumar M, Thapa S, Prajapati U, TakY, et al. Garlic (Allium sativum L.) bioactives and its role in alleviating oral pathologies. Antioxidants (Basel). 2021;10(11):1847. doi:10.3390/antiox10111847.
  12. Weber ND, Andersen DO, North JA, Murray BK, Lawson LD, Hughes BG. In vitro virucidal effects of Allium sativum (garlic) extract and compounds. Planta Med. 1992;58(5):417–23. doi:10.1055/S-2006-961504.
  13. Adetumbi M, Javor GT, Lau BH. Allium sativum (garlic) inhibits lipid synthesis by Candida albicans. Antimicrob Agents Chemother. 1986;30(3):499–501. doi:10.1128/AAC.30.3.499.
  14. Ankri S, Mirelman D. Antimicrobial properties of allicin from garlic. Microbes Infect. 1999;1(2):125–9. doi:10.1016/S1286-4579(99)80003-3.
  15. Slusarenko AJ, Patel A, Portz D. Control of plant diseases by natural products: Allicin from garlic as a case study. Eur J Plant Pathol. 2008;121:313–22. doi:10.1007/s10658-008-9287-x.
  16. Borlinghaus J, Albrecht F, Gruhlke MCH, Nwachukwu ID, Slusarenko AJ. Allicin: chemistry and biological properties. Molecules. 2014;19(8):12591–618. doi:10.3390/molecules190812591.
  17. Martins N, Petropoulos S, Ferreira ICFR. Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: A review. Food Chem. 2016;211:41–50. doi:10.1016/j.foodchem.2016.05.029.
  18. Lanzotti V. The analysis of onion and garlic. J Chromatogr A. 2006;1112(1–2):3–22. doi:10.1016/j.chroma.2005.12.016.
  19. Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y. Intake of garlic and its bioactive components. J Nutr. 2001;131(3 Suppl):955S–62S. doi:10.1093/jn/131.3.955S.
  20. El-Saber Batiha G, Magdy Beshbishy A, Wasef LG, Elewa YHA, Al-Sagan AA, Abd El-Hack ME, et al. Chemical constituents and pharmacological activities of garlic (Allium sativum L.): a review. Nutrients. 2020;12(3):872. doi:10.3390/nu12030872.
  21. Nidadavolu P, Bjarnsholt T, Wu H, Moser C, Hoiby N, Ciofu O. Garlic extract exhibits ntibiofilm activity against Pseudomonas aeruginosa in mouse models of acute and chronic infection. Int J Antimicrob Agents. 2012;40(3):241–6. doi:10.1016/j.ijantimicag.2012.05.005.
  22. Guo N, Wu C, He L, Zhang B, Zhou M, Li M, et al. Allicin enhances the antimicrobial activities of antibiotics on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. J Appl Microbiol. 2015;119(1):45–52. doi:10.1111/jam.12813.
  23. Tsao SM, Yin MC. Enhanced inhibitory effect of cinnamic acid and deferoxamine on PA-I lectin production and biofilm formation in clinical isolates of Pseudomonas aeruginosa by allicin. J Med Microbiol. 2002;51(4):375–80. doi:10.1099/0022-1317-51-4-375.
  24. Cutler RR, Odent M, Hajj-Ali R, Maharjan S, Bennett NJ. In vitro synergy between allicin and antibiotics against isolates of group B Streptococcus. FEMS Immunol Med Microbiol. 2009;55(3):315–20. doi:10.1111/j.1574-695X.2009.00521.x.
  25. El-Saber Batiha G, Magdy Beshbishy A, Tayebwa DS, Shaheen HM, Yokoyama N, Igarashi I. Garlic and its bioactive constituents: A potential candidate in the prevention of cancer by modulating various cell signaling pathways. Anticancer Agents Med Chem. 2021;21(13):1576–85. doi:1 0.2174/1871520621666210121124321.
  26. Argüello-García R, de la Vega-Arnaud M, Loredo-Rodríguez IJ, Mejía-Corona AM, Melgarejo-Trejo E, Espinoza-Contreras EA, Fonseca-Liñán R, González-RoblesA, Pérez-Hernández N, Ortega-Pierres MG. Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis. Front Cell Infect Microbiol. 2018;8:353. doi:10.3389/fcimb.2018.00353.
  27. Fialová J, Roberts SC, Havlíček J. Consumption Of Garlic Positively Affects Hedonic Perception Of Axillary Body Odour. Appetite. 2016;97:8-15. doi:10.1016/j.appet.2015.11.001.
  28. Gatt ME, Strahilevitz J, Sharon N, Lavie D, Goldschmidt N, Kalish Y, et al. A Randomized Controlled Study to Determine the Efficacy of Garlic Compounds In Patients with Hematological Malignancies at Risk for Chemotherapy-Related Febrile Neutropenia. Integr Cancer Ther. 2015;14(5):428-35. doi:10.1177/1534735415588928.
  29. Belguith H, Kthiri F, Ammar AB, Jaafoura H, Hamida JB, Landoulsi A. Morphological and Biochemical Changes of Salmonella hadar Exposed to Aqueous Garlic Extract. Int J Morphol. 2009;27(3):705-13. doi:10.4067/S0717-95022009000300013.
  30. Kaggwa B, Kyeyune H, Munanura EI, Anywar G, Lutoti S, Aber J, et al. Safety and Efficacy of Medicinal Plants Used to Manufacture Herbal Products with Regulatory Approval in Uganda: A Cross-Sectional Study. Evid Based Complement Alternat Med. 2022. doi:10.1155/2022/1304839.
  31. Evidence-Based Complementary and Alternative Medicine. 2022. doi:10.1155/2022/1304839.
  32. Sajali N, Desa MN, Than T, Chong PP. Anti-hyphal formation property of allicin in suppression of Aspergillus fumigatus growth. Malays J Microbiol. 2013;9:245-52. doi:10.21161/mjm.51413.
  33. Naqvi SAZ, Irfan A, Zahoor AF, Zafar M, Maria A, Chand AJ, et al. Determination of antimicrobial and antioxidant potential of agro-waste peels. An Acad Bras Cienc. 2020;92. doi:10.1590/0001-3765202020181103.
  34. Altuntas S, Korukluoglu M. Growth and effect of garlic (Allium sativum) on selected beneficial bacteria. Food Sci Technol. 2019;39(4):897-904. doi:10.1590/fst.10618.
  35. Fonseca GM, Passos TC, Ninahuaman MFML, Caroci AS, Costa LS. Avaliação da atividade antimicrobiana do alho (Allium sativum Liliaceae) e de seu extrato aquoso. RevBras Plantas Med. 2014;16(31):679-84. doi:10.1590/1983-084x/12_150.
  36. Aala F, Yusuf UK, Jamal F, Rezaie S. Antimicrobial effects of allicin and ketoconazole on Trichophyton rubrum under in vitro condition. Braz J Microbiol. 2012;43(2):786-92. doi:10.1590/S1517-83822012000200044.
  37. Betoni JEC, Mantovani RP, Barbosa LN, Stasi LCD, Fernandes Junior A. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Mem Inst Oswaldo Cruz. 2006;101(4):387-90. doi:10.1590/S0074-02762006000400007.
  38. Ledezma E, López J, Marin P, Romero H, Ferrara G, Sousa L, et al. Ajoene in the topical short-term treatment of Tinea cruris and Tinea corporis in humans. Arzneimittelforschung. 2011;49(6):544-7. doi:10.1055/s-0031-1300459.
  39. Ledezma E, Marcano K, Jorquera A, Sousa L, Padilla M, Pulgar M, et al. Efficacy of ajoene in the treatment of tinea pedis: a double-blind and comparative study with terbinafine. J Am Acad Dermatol. 2000;43(5):829-32. doi:10.1067/mjd.2000.107243.
  40. Eja ME, Asikong BE, Abriba C, Arikpo GE, Anwan EE, Enyi-Idoh KA. A comparative assessment of the antimicrobial effects of garlic (Allium sativum) and antibiotics on diarrheagenic organisms. Southeast Asian J Trop Med Public Health. 2007;38(2):343-8.
  41. Reiter J, Levina N, van der Linden M, Gruhlke M, Martin C, Slusarenko A. Diallylthiosulfinate (allicin), a volatile antimicrobial from garlic (Allium sativum), kills human lung pathogenic bacteria, including MDR strains, as a vapor. Molecules. 2017;22(10):1711. doi:10.3390/molecules22101711.
  42. Bouabdelli F, Djelloul A, Kaid-Omar Z, Semmoud A. Antimicrobial activity of 22 plants used in urolithiasis medicine in Western Algeria. Asian Pac J Trop Dis. 2012;2:S530-5. doi:10.1016/S2222-1808(12)60215-1.
  43. Heimesaat MM, Mousavi S, Weschka D, Bereswill S. Garlic essential oil as promising option for the treatment of acute campylobacteriosis—results from a preclinical placebo-controlled intervention study. Microorganisms. 2021;9(6):1140.
  44. doi:10.3390/microorganisms9061140.
  45. Bakhshi M, Taheri JB, Shabestari SB, Tânia A, Pahlevan R. Comparison of therapeutic effect of aqueous extract of garlic and nystatin mouthwash in denture stomatitis. Gerodontology. 2011;29(2):e680-4. doi:10.1111/j.1741-2358.2011.00544.x.
  46. Bekut M, Brkić S, Kladar N, Gavari N, Božin B. Garlic clove applied as vaginal suppository: a case report. Complement Ther Med. 2018;39:97-100. doi:10.1016/j.ctim.2018.05.017.
  47. Fujisawa H, Watanabe K, Suma K, Origuchi K, Matsufugi H, Seki T, et al. Antibacterial potential of garlic-derived allicin and its cancellation by sulfhydryl compounds. Biosci Biotechnol Biochem. 2009;73(9):1948-55. doi:10.1271/bbb.90096.
  48. Ebrahimy F, Dolatian M, Moatar F, Majd HA. Comparison of the therapeutic effects of Garcin® and fluconazole on Candida vaginitis. Singapore Med J. 2015;56(10):567-72. doi:10.11622/smedj.2015153.
  49. Brasil. Ministério da Saúde. Monografia da espécie Allium sativum (Alho). [s.l.: s.n.]. Available from: https://www.gov. br/saude/pt-br/acesso-ainformacao/participacao-social/consultas-publicas/2017/arquivos/monografia-allium.pdf.
  50. Oliveira IN, Everton GO, Ferreira ACC, Sousa IB, de Mouchrek AN, Teles AM, et al. Óleo essencial de alho (Allium sativum) como antimicrobiano frente a cepas ATCC de Escherichia coli e Staphylococcus aureus. Rev Processos Químicos. 2018. doi:10.19142/rpq.v12i23.435.
  51. Pedrosa YS, Araújo JB, de Andrade HH. Avaliação da atividade antifúngica de Allium sativum L. contra Candida albicans. Rev Multidiscip Educ Meio Amb. 2021. doi:10.51189/rema/2647.
  52. Vargas LRC, Mamani JCM, Alvarez EV, Rebollo MS, Romero B. Función antimicrobiana de la alicina de ajo en cultivos de Staphylococcus aureus, Pseudomonas aeruginosa y Escherichia coli. Rev Cient Cienc Méd. 2014;17(1):26-8.
  53. Abidullah M, Jadhav P, Sujan SS, Shtimanikandan AG, Reddy CR, Wasan RK. Potential antial efficacy of garlic extract bacteria on Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae: an in vitro study. J Pharm Bioallied Sci. 2021;13(5):590. doi:10.4103/jpbs.jpbs_681_20.
  54. Farbman KS, Barnett ED, Bolduc GR, Klein JO. Antibacterial activity of garlic and onions. Pediatr Infect Dis J. 1993;12(7):613. doi:10.1097/00006454-199307000-00013.
  55. Acidulas M, Jadhav P, Sujan SS, Shtimanikandan AG, Reddy CR, Wasan RK. Potential antial efficacy of garlic extract bacteria on Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae: an in vitro study. J Pharm Bioallied Sci. 2021;13(5):590. doi:10.4103/jpbs.jpbs_681_20.
  56. Ŝlabur JS, Brajer M, Voća S, Galić A, Radman S, Rimac-Brnčić S, et al. Ultrasound as a promising tool for the green extraction of specialized metabolites from some culinary spices. Molecules. 2021;26(7):1866. doi:10.3390/molecules26071866.
  57. Groppo FC, Ramacciato JC, Simões RP, Flório FM, Sartoratto A. Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms. Int Dent J. 2002;52(6):433-7. doi:10.1111/j.1875.

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